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gfp expression vector pegfp n1 c1  (TaKaRa)


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    TaKaRa gfp expression vector pegfp n1 c1
    Gfp Expression Vector Pegfp N1 C1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/expression+vector+pegfp+c1+n1/pmc11130180-328-5-9
    Average 86 stars, based on 1 article reviews
    gfp expression vector pegfp n1 c1 - by Bioz Stars, 2026-09
    86/100 stars

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    Transfection:

    Article Title: Downregulation of ZBTB24 hampers the G0/1- to S-phase cell-cycle transition via upregulating the expression of IRF-4 in human B cells.
    Article Snippet: Goat polyclonal anti-IRF-4 (M-17, Santa Cruz, Dallas, TX, USA), mouse monoclonal anti-Bimp-1 (C-7, Santa Cruz), anti-β-actin (6G3, Liankebio, Hangzhou, China), Alexa Fluor 680-coupled donkey anti-goat IgG (H+L, 705-625-147) and goat anti-mouse IgG (H+L, 115-625-146) (both from Jackson ImmunoResearch, West Grove, PA, USA) were used in western blotting analysis. .. Plasmids and transfections The complementary DNAs coding for human ZBTB24 and BCL-6 were subcloned into the expression vector pEGFP-C1/N1 (Clontech, Mountain View, CA, USA) p3xFLAG-CMV-10 (Sigma) and pcDNA 3.1/myc-His C. Control or pGL3 vector with 5xBCL6 binding sequences inserted into the XhoI site were kindly provided by Dr Vivan J Bardwell (University of Minnesota, Minneapolis, MN, USA).26 The pcDNA4-BCOR-Myc vector was kindly provided by Dr Herbert C Morse III (National Institutes of Health, Bethesda, MD, USA).27 HEK-293T cells were (co)transfected with expression vectors with Polyjet reagents following the protocols provided by the manufacturer (SignaGen, Changzhou, China). .. Lentiviral transductions To knock down endogenous ZBTB24 expression, lentiviral supernatants were generated by transfection of HEK-293T cells with the pLKD-CMV-GFPU6-shRNA vector (NeuronBiotech, Shanghai, China) containing two different shRNAs targeting human ZBTB24 (ZB24.10: 5′-CAGTGGTTGTT ATCTCTAATA-3′ and ZB24.11: 5′-CCAGTAGTGAATACTTCTCAA-3′).

    Expressing:

    Article Title: Downregulation of ZBTB24 hampers the G0/1- to S-phase cell-cycle transition via upregulating the expression of IRF-4 in human B cells.
    Article Snippet: Goat polyclonal anti-IRF-4 (M-17, Santa Cruz, Dallas, TX, USA), mouse monoclonal anti-Bimp-1 (C-7, Santa Cruz), anti-β-actin (6G3, Liankebio, Hangzhou, China), Alexa Fluor 680-coupled donkey anti-goat IgG (H+L, 705-625-147) and goat anti-mouse IgG (H+L, 115-625-146) (both from Jackson ImmunoResearch, West Grove, PA, USA) were used in western blotting analysis. .. Plasmids and transfections The complementary DNAs coding for human ZBTB24 and BCL-6 were subcloned into the expression vector pEGFP-C1/N1 (Clontech, Mountain View, CA, USA) p3xFLAG-CMV-10 (Sigma) and pcDNA 3.1/myc-His C. Control or pGL3 vector with 5xBCL6 binding sequences inserted into the XhoI site were kindly provided by Dr Vivan J Bardwell (University of Minnesota, Minneapolis, MN, USA).26 The pcDNA4-BCOR-Myc vector was kindly provided by Dr Herbert C Morse III (National Institutes of Health, Bethesda, MD, USA).27 HEK-293T cells were (co)transfected with expression vectors with Polyjet reagents following the protocols provided by the manufacturer (SignaGen, Changzhou, China). .. Lentiviral transductions To knock down endogenous ZBTB24 expression, lentiviral supernatants were generated by transfection of HEK-293T cells with the pLKD-CMV-GFPU6-shRNA vector (NeuronBiotech, Shanghai, China) containing two different shRNAs targeting human ZBTB24 (ZB24.10: 5′-CAGTGGTTGTT ATCTCTAATA-3′ and ZB24.11: 5′-CCAGTAGTGAATACTTCTCAA-3′).

    Control:

    Article Title: Downregulation of ZBTB24 hampers the G0/1- to S-phase cell-cycle transition via upregulating the expression of IRF-4 in human B cells.
    Article Snippet: Goat polyclonal anti-IRF-4 (M-17, Santa Cruz, Dallas, TX, USA), mouse monoclonal anti-Bimp-1 (C-7, Santa Cruz), anti-β-actin (6G3, Liankebio, Hangzhou, China), Alexa Fluor 680-coupled donkey anti-goat IgG (H+L, 705-625-147) and goat anti-mouse IgG (H+L, 115-625-146) (both from Jackson ImmunoResearch, West Grove, PA, USA) were used in western blotting analysis. .. Plasmids and transfections The complementary DNAs coding for human ZBTB24 and BCL-6 were subcloned into the expression vector pEGFP-C1/N1 (Clontech, Mountain View, CA, USA) p3xFLAG-CMV-10 (Sigma) and pcDNA 3.1/myc-His C. Control or pGL3 vector with 5xBCL6 binding sequences inserted into the XhoI site were kindly provided by Dr Vivan J Bardwell (University of Minnesota, Minneapolis, MN, USA).26 The pcDNA4-BCOR-Myc vector was kindly provided by Dr Herbert C Morse III (National Institutes of Health, Bethesda, MD, USA).27 HEK-293T cells were (co)transfected with expression vectors with Polyjet reagents following the protocols provided by the manufacturer (SignaGen, Changzhou, China). .. Lentiviral transductions To knock down endogenous ZBTB24 expression, lentiviral supernatants were generated by transfection of HEK-293T cells with the pLKD-CMV-GFPU6-shRNA vector (NeuronBiotech, Shanghai, China) containing two different shRNAs targeting human ZBTB24 (ZB24.10: 5′-CAGTGGTTGTT ATCTCTAATA-3′ and ZB24.11: 5′-CCAGTAGTGAATACTTCTCAA-3′).

    Binding Assay:

    Article Title: Downregulation of ZBTB24 hampers the G0/1- to S-phase cell-cycle transition via upregulating the expression of IRF-4 in human B cells.
    Article Snippet: Goat polyclonal anti-IRF-4 (M-17, Santa Cruz, Dallas, TX, USA), mouse monoclonal anti-Bimp-1 (C-7, Santa Cruz), anti-β-actin (6G3, Liankebio, Hangzhou, China), Alexa Fluor 680-coupled donkey anti-goat IgG (H+L, 705-625-147) and goat anti-mouse IgG (H+L, 115-625-146) (both from Jackson ImmunoResearch, West Grove, PA, USA) were used in western blotting analysis. .. Plasmids and transfections The complementary DNAs coding for human ZBTB24 and BCL-6 were subcloned into the expression vector pEGFP-C1/N1 (Clontech, Mountain View, CA, USA) p3xFLAG-CMV-10 (Sigma) and pcDNA 3.1/myc-His C. Control or pGL3 vector with 5xBCL6 binding sequences inserted into the XhoI site were kindly provided by Dr Vivan J Bardwell (University of Minnesota, Minneapolis, MN, USA).26 The pcDNA4-BCOR-Myc vector was kindly provided by Dr Herbert C Morse III (National Institutes of Health, Bethesda, MD, USA).27 HEK-293T cells were (co)transfected with expression vectors with Polyjet reagents following the protocols provided by the manufacturer (SignaGen, Changzhou, China). .. Lentiviral transductions To knock down endogenous ZBTB24 expression, lentiviral supernatants were generated by transfection of HEK-293T cells with the pLKD-CMV-GFPU6-shRNA vector (NeuronBiotech, Shanghai, China) containing two different shRNAs targeting human ZBTB24 (ZB24.10: 5′-CAGTGGTTGTT ATCTCTAATA-3′ and ZB24.11: 5′-CCAGTAGTGAATACTTCTCAA-3′).



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